MOLECULAR BIOLOGY

-Bacterial genome sequencing;

-Exploration of the biodiversity of complex microbial communities with the use of metagenomics approaches;

-Bioinformatics of bacterial genomes;

-Deposit of genome sequences and DNA microarray data on public databases;

-In silico analyses of microbial composition of complex microbial communities;

-Transcriptomics analyses through the use of qRT-PCR and microarray technologies;

-Transcriptomic analyses through RNAseq;

-Molecular detection of bacterial strains and identification through the use of PCR strategies;

-DNA quantification by real-time PCR;

-Microbial typing by Comparative Genomes Hybridization assay;

- Microbial typing through PCR based techniques;

-Taxonomic classification of bacteria by 16S rRNA gene sequencing.

 

MICROBIOLOGY

-Isolation of probiotic bacteria;

-Antibiotic susceptibility/resistance;

-Stress response profiling;

-Evaluation of the tolerance to different gut environmental stress (resistance to gastric juices, bile salts; pancreatic juices);  

-Carbohydrate fermentative profiles;

-Evaluation of immune system modulation using in vitro tests;

-Probiotic stability assessment at different storage conditions;

-Adhesion assays to human intestinal monolayers;

-Deposit of strains on International Bacteria Culture Collections.

 

HOST-MICROBE INTERACTIONS

-Evaluation of gut colonization and persistence in murine models;

-Evaluation of the global genome transcription profiling of probiotic bacteria in murine gut;

-Changes in the transcriptome of probiotic bacteria in vitro models.