GenProbio srl offers a complete set of services including both basic microbiological tests as well as advanced molecular biology techniques.
-Bacterial genome sequencing;
-Exploration of the biodiversity of complex microbial communities with the use of metagenomics approaches;
-Bioinformatics of bacterial genomes;
-Deposit of genome sequences and DNA microarray data on public databases;
-In silico analyses of microbial composition of complex microbial communities;
-Transcriptomics analyses through the use of qRT-PCR and microarray technologies;
-Transcriptomic analyses through RNAseq;
-Molecular detection of bacterial strains and identification through the use of PCR strategies;
-DNA quantification by real-time PCR;
-Microbial typing by Comparative Genomes Hybridization assay;
- Microbial typing through PCR based techniques;
-Taxonomic classification of bacteria by 16S rRNA gene sequencing.
-Isolation of probiotic bacteria;
-Stress response profiling;
-Evaluation of the tolerance to different gut environmental stress (resistance to gastric juices, bile salts; pancreatic juices);
-Carbohydrate fermentative profiles;
-Evaluation of immune system modulation using in vitro tests;
-Probiotic stability assessment at different storage conditions;
-Adhesion assays to human intestinal monolayers;
-Deposit of strains on International Bacteria Culture Collections.
-Evaluation of gut colonization and persistence in murine models;
-Evaluation of the global genome transcription profiling of probiotic bacteria in murine gut;
-Changes in the transcriptome of probiotic bacteria in vitro models.